IHMF: Key Paper Bibliography

This section is an invaluable resource, aimed particularly at health professionals.

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Homing in on the cellular immune response to HSV-2 in humans.

Koelle DM, Gonzalez JC, Johnson AS. Am J Reprod Immunol 2005; 53: 172–181

PROBLEM: Genital herpes simplex infections are generally limited to epithelia and neurons. Vaccines have had activity in herpes simplex virus (HSV)-seronegative women only. Understanding how HSV-specific T-cells traffic to infected sites may assist in vaccine design. METHOD OF STUDY: Herpes simplex virus epitopes recognized by HSV-specific CD8 T-cells were identified and used to make fluorescent human leukocyte antigen (HLA)-peptide tetramers. Molecules related to lymphocyte rolling adhesion were studied by flow cytometry and cell binding. HSV-specific CD4 T-cells identified ex vivo by cytokine accumulation or activation marker expression, or detected in vitro by 5-(and-6)-carboxyfluoresce in diacetate, succinimidyl ester (CFSE) dilution, were similarly investigated. RESULTS: Herpes simplex virus-specific T-cells are 10-to 100-fold more prevalent in lesional skin compared with blood and greatly enriched in lesions compared with normal skin. Diverse viral antigens are recognized by HSV-specific T-cells. Functionally active E-selectin ligand, and cutaneous lymphocyte antigen (CLA), are expressed by circulating HSV-2-specific CD8 cells. CD4 cells display lower levels of CLA that are dramatically up-regulated upon re-stimulation with antigen. CONCLUSIONS: Herpes simplex virus-2-specific CD8 and CD4 T-cells differ in constitutive expression of skin homing molecules. Vaccines designed to induce proper homing are postulated to have increased efficacy. Copyright 2005 Blackwell Munksgaard.

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Last Updated : 28/07/2005 16:02:24